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, 2001), and scrambled OPNi 840 were used as negative controls.
Cells were lysed 72 hrs after transfection.
J) NHDF and C13NJ cells were methanol fixed and double labelled while using antibodies anti Sec16B (883–.
897) detected some puncta in NHDF cells, these wouldn't localize in proximity of Sec31A (zoom, arrows).
Efficient depletion of Sec16A may be detected in HeLa control cells and HeLa GFP Sec16B cells (top row, lane 3 and 6).
6 assays were conducted every experiment.
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